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M9490694.TXT
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1994-09-24
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Document 0694
DOCN M9490694
TI Magic Lite Chlamydia immunoassay in urogenital samples and urine versus
chlamydial culture.
DT 9411
AU Bygdeman SM; Johansson M; Jonasson J; Lidbrink P; Nilsson U; Teichert C;
Viktorsson S; Karolinska Institute, Department of Clinical
Bacteriology,; Huddinge Hospital, Stockholm, Sweden.
SO Int J STD AIDS. 1994 May-Jun;5(3):207-11. Unique Identifier : AIDSLINE
MED/94339225
AB Magic Lite Chlamydia assay (commercial test kit for the identification
of Chlamydia trachomatis) was evaluated on urogenital samples and urine
with chlamydial culture as the reference method. The sediment of the
transportation buffer of specimens which were Magic Lite positive but
culture negative or toxic was investigated for elementary bodies with
fluorescein-labelled anti-chlamydial antibodies. The prevalence of
chlamydial infection among the 577 men investigated was 13.7% as
estimated by culture and direct immunofluorescence and 6.4% among the
173 women. In order to improve the sensitivity a cut-off value below
that recommended by the manufacturer was used. The sensitivity of Magic
Lite in male urethral specimens was then 60.8% and that in female
urethral/cervical specimens 90.9%. The specificity was 99.6% and 100%,
respectively. In urine specimens the sensitivity of Magic Lite was 63.3%
(men) and 63.6% (women). The specificity was 99.4% and 100%,
respectively. The sensitivity of Magic Lite on male urethral specimens
was related to the number of inclusion bodies per well in culture and it
was higher among men attending with clinical findings of urethritis
(69%) than among asymptomatic men sampled as a screening procedure (36%)
(P < 0.05). Corresponding differences between the sexes and between
those with and without symptoms were not noted for Magic Lite applied on
urine samples.
DE Adolescence Adult Aged Chlamydia trachomatis/IMMUNOLOGY/*ISOLATION &
PURIF Chlamydia Infections/EPIDEMIOLOGY/*MICROBIOLOGY Evaluation
Studies Female Fluorescent Antibody Technique Human Male Middle Age
Predictive Value of Tests Prevalence Reagent Kits,
Diagnostic/*STANDARDS Sensitivity and Specificity Urine/*MICROBIOLOGY
Urogenital System/*MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).